Bioinformatics Web Practical

It is an online practical for the prediction of the structure and function of the unknown protein by using primary and secondary biological databases
First go http://umber.embnet.org/dbbrowser/bioactivity/
press "ready to go" and then "go" now you are at the page http://umber.embnet.org/dbbrowser/bioactivity/nucleicfrm.html at the top of the you have Sequence translation & identification select "materials" which contain the unknown nucleotide sequences you can also use your own sequence of interest click materials and select any fragment it is the dna sequence click the fragment and get its sequence copy this sequence and paste it in translator to get the translated sequence and also find the orf(open reading frame) of the sequence . Copy the orf and paste in OWL which is actually contain information to which organism your query sequence is present. Find the exact match of your sequence.After finding the exact match copy the name of the organism and paste in query at http://umber.embnet.org/dbbrowser/bioactivity/proteinfrm.html to get the full protein sequence to which our query is part.After getting the full sequence copy it and paste in psi blast to get similarity to other related proteins
now you can find the structure and function of the protein by using this primary database the secondary database procedure will be told you latter. The first hit of the blast result will be the sequence of our query protein you can find its structure and function and compare its structure and function to other hits.