How to use Genbank Database

The Genbank Sequence Database is an open access,annotated collection of all publically available sequences and their protein translations. This database is produced at NCBI as the part of INSDC .

For making use of Genbank follow this tutorial:
Making sense of the Genbank entry of the Prokaryotic Gene.
Go to:
www.ncbi.nlm.nih.gov/entrez/.
Select Nucleotide from search drop down menu and enter your query's accession number from Genbank. eg accession number is X01714 .The output page will be displayed which will tell you E.coli dut gene for dUTPase.
Then click the test button on the text bar to generate a true flat file format of the entry and you can save your entry
by choosing file save from your browser's main menu.
click on the hyperlink for further details of the entry.

the Genbank file format has three parts
1. Comment or defination line
2. Citation
3. Sequence
You can also view the Fasta format of the gene which includes the defination line and nucleotide sequence of your query.
from display settings select FASTA.
To go further retrieving Genbank  entries without using accession numbers.
 Select nucleotide from search drop down menu and type your query in the form of keywords as in the case of
 Human[organism] AND dUTPase [protein name].
Accession number search will give you the exact result of gene on locus but keyword search will give you many entries like exon 1,exon 2,exon 3, mRNA 1,mRNA 2 etc. different accession number entries indicate full amino acid sequence of the two forms of(nuclear and mitochondrial) of the dUTPase protein as well as alternative exon usage pattern.
 This tutorial will over I hope you will be benifited from this tutorial.
Regards "Quratt ul ain Siddique"