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Saturday, October 30, 2010

Muliple Sequence Alignment

For Multiple sequence Alignment you need Fasta sequence for this go to ncbi for nucleotide or at expasy server for protein sequence but now I am going to work with proteins so go to www.expasy.org
enter your query for which you want to retrieve the sequences
or you can also go to uniprot which in my opinion is easy to use and get fasta sequence so go to uniprot and enter protein hemoglobin and get different entries of this protein click them and get fasta sequence like this

and select many proteins and get their fasta formats like this
 click on fasta and http://www.uniprot.org/uniprot/P68871.fasta
copy and paste these fasta sequences of many proteins in a text file and collect all these sequences in a text document.
In this format I pasted my all sequences in my notepad

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