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Saturday, November 27, 2010

Rasmol:gcufbioinfo

Click on different balls of your molecule and it will give information about the particular atom you clicked in which it exists.

rasmol:gcufbioinfo

open raswin and select file>information to get  complete information about the particular molecule.

Rasmol:gcufbioinfo

Select raswin from your list and open it select file>open and select file from any derive and open it into rasmol .
click Display>and select different options to view your molecule.

Rasmol:gcufbioinfo

This was the way in which you can visualize the molecule using Rasmol on the pdb page but if you want to view the saved page of pdb so click the option "Display files".
And save this file to any directory with file format ".pdb" so now you can visualize already saved molecule.

Rasmol:gcufbioinfo

To view Structure using Rasmol you must have pdb file of your macromolecule and micromolecule so go to PDB
enter protein name for which you want to view the structure. As I entered DNA Polymerase so the result is http://www.pdb.org/pdb/explore/explore.do?structureId=3OGU
the out put is as:
 At the right of this page you can there is the option of Download files so click this. After clicking this select "PDB files(gz)".then download and install winzip to your system and download this file automatically to raswin and view the structure in rasmol.

What kinds of molecules can RasMol display?:Gcufbioinfo

RasMol can display any molecule for which a 3-dimensional structure is available. 3D structures have not been determined for many molecules of great interest; these, RasMol cannot display.
In order to display a molecule, RasMol needs a data file called an atomic coordinate file. This data file specifies the position of every atom in the molecule, as cartesian coordinates X, Y, and Z.
Three-dimensional structures can be predicted for many small molecules, but must be determined empirically for macromolecules. The most common method for determining structure is X-ray diffraction analysis of a crystal. Nuclear magnetic resonance (NMR) can also be used. Some structures are available only as theoretical models, often based on related molecules for which empirical structures have been determined.

download rasmol:GCUFBIOINFO


rasmol - Full Download    

rasmol [2010

rasmol Free Download
[COMPLETE] - rasmol

rasmol [FullVersion]
 
                                                                                        

Rasmol Tool:

RasMol is a computer program written for molecular graphics visualization intended and used primarily for the depiction and exploration of biological macromolecule structures, such as those found in the Protein Data Bank. It was originally developed by Roger Sayle in the early 90s  get started this useful tool Download Rasmol 
here. Install Rasmol to your system and get started.

Thursday, November 25, 2010

HTML Calculator:gcufbioinfo

paste this code to your source code of your calculator in head part
function sqrt(form)
 {
form.displayvalue.value=Math.sqrt(form.displayvalue.value);
 }
function sq(form)
 {
form.displayvalue.value=eval(form.displayvalue.value)*eval(form.displayvalue.value);
 }
function ln(form)
 {
form.displayvalue.value=Math.exp(form.displayvalue.value);
 }
function equal(form)
 {
form.displayvalue.value=eval(form.displayvalue.value);
 }
function delChar(input)
 {
input.value=input.value.substring(0,input.value-length-1);
 }
function addChar(input,character)
 {
if(input.value==null||input.value=="0")
      input.value=character
else
      input.value+=character
 }
Insha Allah you will see your functioning very soon regards Admin Quratt ul ain Siddique.

Monday, November 22, 2010

HTML Calculator:gcufbioinfo

Add this code to your code of calculator in head portion
<head>
<script type="text/javascript">
function cos(form)
 {
form.displayvalue.value=Math.cos(form.displayvalue.value);
 }
function sin(form)
 {
form.displayvalue.value=Math.sin(form.displayvalue.value);
 }
function tan(form)
 {
form.displayvalue.value=Math.tan(form.displayvalue.value);
 }

</script>
</head>
I will tell you other codes as soon as possible and you will see Insha Allah your calculator functional

HTML Calculator:gcufbioinfo

As I told you how to generate calculator using html codes so this is again calculator with some changes so plz try it. I will tell you how to make it functional
<html>
<title>Calculator</title>
<head></head>
<body>
<table border="2">
<tr>
<td><input name="displayvalue" size="39" value="0"></td>
   <tr>
     <td>
         <table border="2">
          <tr>
            <td>
                 <input type="button" value="     char      ">
            </td>
        <td>
        <input type="button" value="     delete       ">
        </td>
              <td>
        <input type="button" value="        =         ">
        </td>
          </tr>
         </table>
     </td>
            <tr>
              <td>
           <table border="2">
                <tr>
                <td>
                  <input type="button" value="  exp  ">
                </td>
                <td>
                  <input type="button" value="    7    ">
                </td>
                <td>
                  <input type="button" value="    8    ">
                </td>
                <td>
          <input type="button" value="    9    ">
        </td>
                <td>
                  <input type="button" value="  /  ">
                </td>
                </tr>
                <tr>
                <td>
                <input type="button" value="    ln    ">
      
                </td>
                <td>
                        <input type="button" value="    4    ">
                </td>
                <td>
                        <input type="button" value="   5     ">
                </td>
                <td>
            <input type="button" value="   6     ">
                </td>
                <td>
            <input type="button" value="  *  ">
                </td>
                </tr>
                <tr>
                 <td>
            <input type="button" value="  sqrt  ">
                 </td>
                 <td>
                        <input type="button" value="    1    ">
                 </td>
                 <td>
            <input type="button" value="    2    ">
                 </td>
                 <td>
            <input type="button" value="    3    ">
                 </td>
                 <td>
            <input type="button" value="  -  ">
                 </td>
                </tr>
                <tr>
                 <td>
            <input type="button" value="   sq   ">
                 </td>
                 <td>
            <input type="button" value="    0    ">
                 </td>
                 <td>
            <input type="button" value="     .    ">
                 </td>
                 <td>
            <input type="button" value="   %   ">
                 </td>
                 <td>
                        <input type="button" value="  + ">
                 </td>
                </tr>
                <tr>
                 <td>
              <input type="button" value="     (     ">
                 </td>
                 <td>
            <input type="button" value="  cos ">
         </td>
                 <td>
            <input type="button" value="  sin   ">
                 </td>
                 <td>
            <input type="button" value="  tan  ">
                 </td>
                 <td>
            <input type="button" value="  )  ">
                 </td>
                </tr>
           </table>
              </td>
        </tr>
   </tr>

</tr>
</table>
</body>
</html>
out put is as:
Now I tell you how to make it functional
Quratt ul ain Siddique

Sunday, November 21, 2010

imageJ:gcufbioinfo

Open your image in imageJ and select adjust in imageJ adjust>color and contrast you can use these sliders to adjust display range, either down from the top or up from the bottom.

Medical Image Processing tool:

Now we are going to see how to view an image using imageJ on gcufbioinfo.
First save an image especially a medical image to your system and copy this image and go to C derive then program files and then imagej folder and paste this image into it. then open imageJ from start menu and click file >open as shown in gcufbioinfo and select the picture to view.
you can view your image using this tool .

imageJ:gcufbioinfo

You can access imageJ by clicking on start button and click imageJ to click the microscope icon. The out put you can see at gcufbioinfo and click help to get information about imageJ.

Download ImageJ:

To download imageJ first go to the http://rsbweb.nih.gov/ij/
the page will open

click download
http://rsbweb.nih.gov/ij/download.html
then get latest version of imageJ for your OS . If you are new to use ImageJ then Download ImageJ 1.43 bundled with 32-bit Java 1.6.0_10 (25MB) other wise download without java for windows OS.

Medical Image Processing tool:

ImageJ:
ImageJ is a public domain, Java-based image processing program developed at the National Institutes of Health. ImageJ was designed with an open architecture that provides extensibility via Java plugins and recordable macros. Custom acquisition, analysis and processing plugins can be developed using ImageJ's built-in editor and a Java compiler. User-written plugins make it possible to solve many image processing and analysis problems, from three-dimensional live-cell imaging, to radiological image processing,multiple imaging system data comparisonsto automated hematology systems.ImageJ's plugin architecture and built in development environment has made it a popular platform for teaching image processing.

Tuesday, November 2, 2010

peptide bond:

Amino Acids:

Dancing protein:

protein structure:

primary structure analysis:

Tool:
Radar:De novo repeat detection in protein sequences 
 I entered the protein albumin in uniprot and get the following result It gave the number of repeats present in  the sequence.
http://www.ebi.ac.uk/Tools/es/cgi-bin/jobresults.cgi/radar/radar-20101102-1505421645.html
 

Monday, November 1, 2010

primary structure analysis

Tools: 
Radar :De novo repeat detection in protein sequences go to http://www.ebi.ac.uk/Tools/Radar/index.html

primary structure analysis:

Tools: 
HeliQuest A web server to screen sequences with specific alpha-helical properties HeliQuest calculates from an α-helix sequence its physicochemical properties and amino acid composition and uses the results to screen any databank in order to identify protein segments possessing similar features.
The server is divided into 2 interconnected modules: the sequence analysis module and the screening module.
In addition, the mutation module, (available from the sequence analysis module), allows user to mutate helices manually or automatically by genetic algorithm to create analogues with specific properties.-http://heliquest.ipmc.cnrs.fr/
After entering the helix sequence of the protein this protein sequence is get by entering the name of the protein in uniprot then paste it by clicking the analysis button and get the following
http://heliquest.ipmc.cnrs.fr/cgi-bin/ComputParams.py

Tools: 
ScanSite pI/Mw:Compute the theoretical pI and Mw, and multiple phosphorylation states. Enter a protein name and sequence below. The molecular weight and isoelectric point of this sequence and multiple phosphorylation states will be displayed. http://scansite.mit.edu/calc_mw_pi.html
out put will be

primary structure analysis 3:

Tool: 
Compute pI/Mw: Compute the theoretical isoelectric point (pI) and molecular weight (Mw) from a UniProt Knowledgebase entry or for a user sequence
now go to this tool and get the required result Please enter one or more UniProtKB/Swiss-Prot protein identifiers (ID) (e.g. ALBU_HUMAN) or UniProt Knowledgebase accession numbers (AC) (e.g. P04406), separated by spaces, tabs or newlines. Alternatively, enter a protein sequence in single letter code. The theoretical pI and Mw (molecular weight) will then be computed. previosly I entered the amino acid sequence and now I am going to enter the uniprot AC#.http://www.expasy.org/cgi-bin/pi_tool
it will give you this format of result so click on the following things according to the instructions mentioned Please select one of the following features by clicking on a pair of endpoints, and the computation will be carried out for the corresponding sequence fragment.

Primary structure analysis 2:

Tool :
ProtParam :Physico-chemical parameters of a protein sequence (amino-acid and atomic compositions, isoelectric point, extinction coefficient, etc.)
First go to this page http://www.expasy.org/tools/ select  Primary structure analysis and select the first tool as  I mentioned. click the tool and I entered the amino acid sequence of protein transgelin-2 in  http://www.expasy.org/tools/protparam.html then get the out put page
http://www.expasy.org/cgi-bin/protparam
the out put is

Primary structure analysis:

Now we are going to the expasy server for the Primary structure analysis of proteins by using different tools available at expasy server.
I will breifly tell you how to use these tools.

HTML Calculator:

As I told you how to generate a table in html today I am going to develop a calculator by using html table tags but the  new things is the button so the code for the button is
<input type="button" value="submit">
<html>
<body>
<input type="button" value="submit">
<br><br>
<table border="2">
<tr><td><input type="text"></td></tr>
<tr><table border="2"><tr><td><input type="button" value="1"></td><td><input type="button" value="2"></td><td><input type="button" value="3"></td><td><input type="button" value="c"></td></tr><tr><td><input type="button" value="4"></td><td><input type="button" value="5"></td><td><input type="button" value="6"></td><td><input type="button" value="/"></td></tr><tr><td><input type="button" value="7"></td><td><input type="button" value="8"></td><td><input type="button" value="9"></td><td><input type="button" value="*"></td></tr><tr><td><input type="button" value="0"></td><td><input type="button" value="."></td><td><input type="button" value="="></td><td><input type="button" value="-"></td></tr><tr><td><input type="button" value="+"></td><td><input type="button" value="%"></td><td><input type="button" value="d"></td><td><input type="button" value="f"></td></tr></table></tr>
</table>
</body>
</html>
this is not a functioning calculator it is just a sekeleton of calculator the functioning of this calculator I will told you latter 
regards Admin Quratt ul ain Siddique 

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