primary structure analysis 3:

Tool: 
Compute pI/Mw: Compute the theoretical isoelectric point (pI) and molecular weight (Mw) from a UniProt Knowledgebase entry or for a user sequence
now go to this tool and get the required result Please enter one or more UniProtKB/Swiss-Prot protein identifiers (ID) (e.g. ALBU_HUMAN) or UniProt Knowledgebase accession numbers (AC) (e.g. P04406), separated by spaces, tabs or newlines. Alternatively, enter a protein sequence in single letter code. The theoretical pI and Mw (molecular weight) will then be computed. previosly I entered the amino acid sequence and now I am going to enter the uniprot AC#.http://www.expasy.org/cgi-bin/pi_tool
it will give you this format of result so click on the following things according to the instructions mentioned Please select one of the following features by clicking on a pair of endpoints, and the computation will be carried out for the corresponding sequence fragment.